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For each sequencing platform, filled points indicate the number of ASVs with an exact match to a theoretical ASV, while open points represent the total number of ASVs. For each primer set, the dotted line marks the number of theoretical ASVs based on the curated <t>Zmock</t> reference sequences trimmed to match the amplified regions. Identical subsampling depths were applied across all datasets, and the final point represents the maximum number of available reads.
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For each sequencing platform, filled points indicate the number of ASVs with an exact match to a theoretical ASV, while open points represent the total number of ASVs. For each primer set, the dotted line marks the number of theoretical ASVs based on the curated <t>Zmock</t> reference sequences trimmed to match the amplified regions. Identical subsampling depths were applied across all datasets, and the final point represents the maximum number of available reads.
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For each sequencing platform, filled points indicate the number of ASVs with an exact match to a theoretical ASV, while open points represent the total number of ASVs. For each primer set, the dotted line marks the number of theoretical ASVs based on the curated <t>Zmock</t> reference sequences trimmed to match the amplified regions. Identical subsampling depths were applied across all datasets, and the final point represents the maximum number of available reads.
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For each sequencing platform, filled points indicate the number of ASVs with an exact match to a theoretical ASV, while open points represent the total number of ASVs. For each primer set, the dotted line marks the number of theoretical ASVs based on the curated Zmock reference sequences trimmed to match the amplified regions. Identical subsampling depths were applied across all datasets, and the final point represents the maximum number of available reads.

Journal: bioRxiv

Article Title: Nanopore sequencing reaches amplicon sequence variant (ASV) resolution

doi: 10.64898/2026.02.26.708165

Figure Lengend Snippet: For each sequencing platform, filled points indicate the number of ASVs with an exact match to a theoretical ASV, while open points represent the total number of ASVs. For each primer set, the dotted line marks the number of theoretical ASVs based on the curated Zmock reference sequences trimmed to match the amplified regions. Identical subsampling depths were applied across all datasets, and the final point represents the maximum number of available reads.

Article Snippet: We first evaluated the ASVs generated from both PacBio and ONT sequencing of the ZymoBIOMICS mock community (Zmock) (Cat. No. D6305, Zymo Research, USA), which contains eight bacteria and two fungal species ( Supplementary Table S1 ).

Techniques: Sequencing, Amplification